Corning® Matrigel® Matrix for Organoids | Disease Modeling and Drug Discovery | Corning

• Verified to support growth and differentiation of mouse and human organoid cultures including:
  • Long-term expansion of mouse small intestinal organoids for more than 7 passages with typical organoid budding morphology and marker expression³
  • Growth and differentiation of polarized 3D organoids from primary human airway epithelial cells expressing typical markers⁴
• Each lot is measured for its elastic modulus, which is indicative of matrix stiffness
• Each lot is qualified to form stable "3D dome" structures commonly used in organoid culture
• Demonstrated to successfully grow organoids from both healthy and diseased cell origins

As an optimized matrix, Corning Matrigel matrix for organoid culture reduces the need for time-consuming screening, while providing the reproducibility and consistency essential for organoid research.

<sup>1.  Hartman CD, et al. Extracellular matrix type modulates cell migration on mechanical gradients. Experimental Cell Research, vol. 359, no. 2, pp. 361-366, 2017.
2.  Bryant DM, et al. A molecular switch for the orientation of epithelial cell polarization. Developmental Cell, vol. 31, no. 2, pp. 171-187, (2014).
3.  Application note: CLS-AN-542, Culture of Mouse Intestinal Organoids in Corning® Matrigel® Matrix for Organoid Culture, K. Slater and H. Nandivada
4. Application note: CLS-AN-534, High Throughput Gene Expression Analysis of 3D Airway Organoids, H. Sherman, N. Elliott, A. White and A. Rossi</sup>

Organoids support advancements in the study of organogenesis, disease modeling, and subsequently patient-specific therapies. Stem cells and/or organ progenitors from normal or diseased tissue are mixed with Corning Matrigel matrix to create mini-organs of the: kidney, thyroid, liver, brain, lung, intestine, prostate, breast, esophagus, gastric, ovarian, and pancreas.

Corning Matrigel matrix is the most published hydrogel for organoid research due to its close resemblance to an in vivo environment, providing necessary growth factors, proteins, and the required matrix architecture.