Matrigel Matrix | Trusted Extracellular Matrices

Overview

Corning Matrigel Matrix

Cells behave better on Corning Matrigel matrix—the original, trusted extracellular matrix (ECM).

Nearly 30 years ago, researchers were looking for a way to grow mouse sarcoma cells. The solution? The development of Corning Matrigel matrix. Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such ECM proteins as laminin (a major component), collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Today, Matrigel, a natural ECM-based hydrogel is among the most widely used models for 2D and 3D cell culture in vitro. Corning matrigel enables you to:

Improve the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid cells, as well as other cell types.
Better mimic in vivo environments for 2D and 3D cell culture applications.

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Matrigel Matrix Handling Tips

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Characteristics of Matrigel Matrix
	Standard Formulation	High Concentration (HC)	Growth Factor Reduced (GFR)	Phenol Red Free	hESC-qualified
Application	Suitable for culture of polarized cells, such as epithelial cells. Promotes differentiation of many cell types, including hepatocytes, neurons, beta-islets, mammary epithelial, endothelial, and smooth muscle cells.	Higher protein concentration provides greater matrix stiffness and scaffold integrity. Suitable for in vivo cell delivery applications for improved cell engraftment and augmentation of solid tumor formation.	Suited for applications where a more highly defined basement membrane preparation is desired. Available in standard, Phenol red-free, and GFR formulations.	Suitable for assays that require color detection (e.g., colorimetric, fluorescence).Available in standard, GFR, and HC formulations.	Pre-screened for compatibility with mTeSR®1 medium by Stem Cell Technologies, providing the reproducibility and consistency essential for human embryonic and induced pluripotent feeder-free stem cell culture.
Source	Mouse	Mouse	Mouse	Mouse	Mouse
Protein Concentration*	8-12 mg/mL	18-22 mg/mL	8-12 mg/mL	8-12 mg/mL	Dilution Factor
Shelf Life	2 years from date of manufacture. Date of expiration is located on a lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on a lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on a lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on a lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on a lot-specific certificate of analysis.
Vialed Formats (Cat. No./Qty.)	356234 \| 5 mL 354234 \| 10 mL 356235 \| 5 x 10 mL 356237 \| 10 mL (Phenol red-free)	354248 \| 10 mL 354262 \| 10 mL (Phenol red-free) 354263 \| 10 mL (GFR)	356230 \| 5 mL (Standard) 354230 \| 10 mL (Standard) 354263 \| 10 mL (HC) 356231 \| 10 mL (Phenol red-free)	356237 \| 10 mL (Standard) 354262 \| 10 mL (HC) 356231 \| 10 mL (GFR)	354277 \| 5 mL
BioCoat^TMOptions	Plates: 6 well, 12 well, 24 well, 48 well, 96 well Inserts: for 24 well plates Dishes: 35 mm, 60 mm, 100 mm

*Protein concentration mentioned above are typical ranges; in some instances, lots may fall outside this range.
See Certificate of analysis for information on individual lots.

Features

Unique features of Matrigel matrix include:

Proven and trusted since 1985; most extensively referenced basement membrane matrix.
Wide selection of basement membranes; used in diverse applications, from cancer and stem cell research to neurobiology.
Manufactured under stringent quality control practices that exceed industry standards, ensuring protein consistency and superior product performance.
Rigorous validation for functionality.
Certified LDEV-free.

Proven and trusted since 1985; most extensively referenced basement membrane matrix.
Wide selection of basement membranes; used in diverse applications, from cancer and stem cell research to neurobiology.
Manufactured under stringent quality control practices that exceed industry standards, ensuring protein consistency and superior product performance.
Rigorous validation for functionality.
Certified LDEV-free.

Cells

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Compatible Cell Types

Primary Cells

Cell Lines (Transformed or Transfected)

Stem and Progenitor Cell Expansion

In Vitro Differentiation of Pluripotent Stem Cells

In Vitro Differentiation of Adult Stem Cells

Aortic endothelial cells, BAEC

Bile duct cells (epithelial)

Brain microvessel (endothelial)

Cardiomyocytes; cardiac (endothelium, progenitor cells)

Colonocytes (epithelial)

Dorsal root ganglia

Embryonic cortical neurons

Embryonic sympathetic neurons

Hepatocytes

Hippocampal neurons

HUVEC (endothelial)

HVSMC

Keratinocytes

Mammary epithelial cells; breast cells (luminal, myoepithelial and endothelial)

Microvascular, BME (endothelial)

Mouse splenic T-Cells

Muscle cells, myoblasts, myogenic cells, myotubes

Oligodendrocytes (glial; precursors)

Pancreatic islet, neonatal (3- to 5-day-old) rat islets of Langerhans

Parotid acinar cells

Peripheral blood mononuclear cells

Schwann cells (glial)

Sertoli cells (spermogenic)

Skeletal muscle cells (myocytes, myotubes)

Smooth muscle cells (endothelial, vascular)

Urothelial cells

Breast cancer cells (established cell lines)

C2C12 (myoblast)

CHO, CHO-1, CHO-K1 (epithelial, endothelial, transfected fusion protein)

COS-7 (fibroblast, transfected)

Dorsal Root Ganglia (transfected)

H1299 (transfected- human non-small cell lung carcinoma cell line)

HEK-293 (transfected, epithelial), EcoPack2™-293, HEK-SRAtet cells, Living Colors HEK-ZsGreen proteasome sensor (transfected)

HepG2 (hepatocyte)

HT-1080 (epithelial)

hFOB 1.19, MG63 (osteoblast cell lines)

L929 (fibroblast, transfected)

LnCAP (prostate cancer cell line)

MCF-10A (epithelial

MDA-MB-231

MDA-MB 435

NIH/3T3, 3T3 (fibroblast)

PC-3, PC-12

RTG-2 (rainbow trout gonad cells)

SH-SY5Y

Human embryonic stem cell (hESC)

Human induced pluripotent stem cell (hiPSC)

hESC (cerebral organoid model)

hESC (pancreatic)

hESC, hiPSC (cardiomyocytes)

hESC, hiPSC, mESC (Germ Cell Layers: ectoderm, mesoderm, endoderm; hematopoietic progenitor; definitive differentiation; cardiomyocytes)

hESC, hiPSC, mESC, miPSC (endothelial)

hESC, hiPSC (intestinal organoids)

hESC, hiPSC (neuronal)

hESC, hiPSC (smooth muscle)

hESC, mESC (lung epithelial)

hESC, mESC, rESC (hepatocyte, hepatocyte-like)

Human NPCs (differentiation to neuronal cells)

hPSCs, mPSCs (renal progenitor cells, renal tubular cells, endoderm)

mESC (hematopoietic)

mESC, Chicken (cardiomyocytes)

mESC, rESC, miPSC (neuronal, progenitor)

mPSCs (inner ear sensory epithelia)

hADSCs; Adipose (endothelial)

Colon (epithelial organoids)

Hair Follicle (melanocytes, neurons, smooth muscle)

Intestinal (organoids, crypt-villus)

Applications for Matrigel Matrix

Corning Matrigel matrix is effective for the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid and other cell types. These include neurons, Sertoli cells, chick lens, vascular endothelial cells, and hepatocytes.

Matrigel Matrix Applications include:

Influencing gene expression in adult rat hepatocytes
3D culture in mouse and human mammary epithelial cells
In vivo peripheral nerve regeneration
Metabolism and toxicology studies
The development of several types of tumor cell invasion assays
The in vitro and in vivo study of angiogenesis
In vivo propagation of human tumors in immunosuppressed mice
The maintenance of human embryonic stem cells and induced pluripotent stem (hiPS) cells
Neuronal differentiation of hiPS cells

Matrigel Matrix Quality Control Specifications

Mouse colonies routinely screened for pathogens via mouse antibody production (MAP) testing
LDEV-free EHS tumor
Extensive PCR testing performed on a number of pathogens, including LDEV, ensuring strict control of raw materials used during manufacturing
Tested and found negative for the presence of bacteria, fungi, and mycoplasma
Protein concentrations determined by Lowry method
Endotoxin units measured by Limulus Amoebocyte Lysate assay
Gel stability tested for a period of 14 days at 37°C
Biological activity determined for each lot using a neurite outgrowth assay; chick dorsal root ganglia, plated on a 1.0 mm layer of Corning Matrigel matrix, required to generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

Using Organoids for Personalized Medicine: An Interview with Sylvia Boj from HUB

Hear from Dr. Sylvia Boj, Chief Research Officer at Hubrecht Organoid Technology (HUB), to learn more about how researchers are using Corning Matrigel Matrix to generate organoid models for personalized medicine research in hopes to find the right treatment for each individual patient.

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Free Download

Request a copy of the Ultimate Guide to Matrigel Matrix – a go-to resource packed with a plethora of resources from top tips and facts to expert advice on Matrigel Matrix.

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Hear more about Dennis Plenker’s and Ping Zhou’s work and how they are using Corning® Matrigel® matrix.

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Dennis Plenker

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Ping Zhou

Applications and Use

Matrigel Matrix in Action

Cancer Research

Corning Matrigel matrix supports significant advances in cancer cell biology.

3D Cell Culture

Sophisticated in vitro 3D cell culture models are having a major impact across many research areas

Disease Modeling

Disease models support our understanding of how diseases develop, progress, and treatment options.

Corning Matribot® Bioprinter Demo Request

Accelerate development and streamline the workflow of 3D models.

Maintenance of Human iPS Cells

Matrigel for culturing human iPS cells in a feeder-free environment.

In vivo Implantation

Use Matrigel matrix in angiogenesis and human tumor implantation in mice.

Celebrate the Past. Imagine the Future.

10,000+ Citations and Counting

Organoid Models

Explore organoid model environments.

Lung Organoids

Lung organoids have utility in the study of infectious human respiratory diseases and lung disorders such as cystic fibrosis and asthma.

Featured Resources

Biological vs. Synthetic ECMs

Consider the compatibilities of the cell type, application, and variability tolerance comparing biological vs. synthetic ECMs with our infographic.

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Corning Matrigel Matrix FAQs

From growth factor concentration to cell recovery and storage, learn how to optimize your cell culture with Corning Matrigel matrix.

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Infographic: Why is Corning Matrigel Matrix so Popular?

Corning Matrigel matrix is the most highly referenced extracellular matrix and it's widely used for 2D and 3D studies, with 13,000+ citations.

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Basics of Corning® Matrigel® Matrix

This webinar introduces basic information on Corning Matrigel matrix and discuss tips and techniques for handling Corning Matrigel matrix.

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Choosing the Right Cell Culture Surface: Modified Surfaces

In this webinar you will learn basic principles behind tissue culture treatment, and when to use enhanced attachment surfaces.

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Choosing the Right Cell Culture Surface: Extracellular Matrix Coatings

In this webinar you will learn key differences between natural, mimetic, and synthetic ECM coatings and applications for specific ECM coatings.

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Why HUB Organoids are Key to Advancing Personalized Cancer Therapies

HUB Organoids details how patient-derived organoids are becoming promising models for personalized treatment using 3D cell culture technologies from Corning & Yamaha Corporation.

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Using Organoids in the Study of Infectious Diseases

Organoid cell culture has transformed cell-based assays in drug discovery and basic biology by conferring physiologic relevance to in vitro cell-based biological models.

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A Life Scientist’s How-To Guide on Corning Matrigel Matrix and Organoids

This guide details best practices to consider for organoid work, as well as how to use Corning Matrigel matrix and other products to support your organ-specific research.

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Guide to 3D Model Systems: Spheroids, Organoids and Tissue Models

Download this guide to 3D models with helpful tips & techniques to set your 3D culture up for success.

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Application Notes

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration

In this paper, we investigated the effect of protein concentration on the elastic moduli of Corning Matrigel® matrix and Collagen I gels using a rotational rheometer.

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Protocol for Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Step-by-step protocol for on-top MDCK 3D culture and embedded MDCK 3D culture.

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A Novel Method for Generating Single, Intestinal Organoids for High Throughput Screening

Here we demonstrate the formation of gastrointestinal organoids derived from human induced pluripotent stem cells (hiPSC) in Corning® 96-well spheroid microplates in combination with Corning Matrigel® matrix, resulting in a single intestinal organoid in each well.

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Methods for Implantation of Corning Matrigel Matrix into Mice and Tissue Fixation

Use Matrigel matrix in angiogenesis and human tumor implantation in mice.

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Maintenance of Human iPS Cells in a Feeder-free Culture System

Corning Matrigel matrix is an effective surface that supports feeder-free expansion and maintenance of undifferentiated human iPS cells.

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View All Matrigel Application Notes

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How to Buy

Purchasing Options

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Order Online

It’s easy to purchase Matrigel matrix online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

It’s easy to purchase Matrigel matrix online. Order direct with a credit card or use a purchase order if you have an account with Corning.

Distributors

Distributors

Contact your authorized distributor to order Matrigel matrix.

Customer Service

Customer Service

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

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Corning Matribot Bioprinter

The first benchtop device to dispense and print Corning Matrigel matrix – and other ECM hydrogels – with ease.

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Matrigel Matrix-3D Plates

Corning is pleased to offer our Matrigel matrix-3D plates, a pre-coated, ready-to-use option available in 96-well and 384-well formats.

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Matrigel Matrix for Organoid Culture

Corning Matrigel Matrix for Organoid Culture is a solubilized basement membrane preparation that has been optimized for organoid culture.

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Matrigel Matrix | Trusted Extracellular Matrices | Corning

Corning® Matrigel® Matrix

Corning Matrigel Matrix

Cells behave better on Corning Matrigel matrix—the original, trusted extracellular matrix (ECM).

Matrigel Matrix Handling Tips

Unique features of Matrigel matrix include:

Unique features of Matrigel matrix include:

Applications for Matrigel Matrix

Matrigel Matrix Quality Control Specifications

Free Download

Hear more about Dennis Plenker’s and Ping Zhou’s work and how they are using Corning® Matrigel® matrix.

Matrigel Matrix in Action

Cancer Research

Cancer Research

3D Cell Culture

3D Cell Culture

Disease Modeling

Disease Modeling

Corning Matribot® Bioprinter Demo Request

Corning Matribot® Bioprinter Demo Request

Maintenance of Human iPS Cells

Maintenance of Human iPS Cells

In vivo Implantation

In vivo Implantation

Celebrate the Past. Imagine the Future.

Celebrate the Past. Imagine the Future.

Organoid Models

Organoid Models

Lung Organoids

Lung Organoids

Featured Resources

Application Notes

Purchasing Options

Order Online

Order Online

Distributors

Distributors

Customer Service

Customer Service

You May Also Be Interested In These Related Products

Connect with Corning

Connect with Corning