Identifying and Correcting Common Cell Growth and Attachment Problems

     Presented By Jeanne Phillips, Ph.D.

     Tuesday, April 13, 2010
     12 noon - 1 pm EST or 17:00 - 18:00 (UK); 18:00 - 19:00 (Europe)

     Thursday, April 15, 2010
     9 am - 10 am EST or 14:00 - 15:00 (UK); 15:00 - 16:00 (Europe)

This seminar will review some of the common and not so common cell culture problems that are often very difficult to identify and eliminate.  If not effectively dealt with, these problems can result in culture loss and erroneous data, disrupt research and lead to personal embarrassment.  Approaches for managing these problems will be discussed.  Special attention will be given to problems with:

• Culture media
• Cell attachment
• Incubators
  

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Primary Cell Culture - Tips and Techniques for Getting Started

     Presented By Jaishree Trikha, Ph.D.

     Tuesday, May 11, 2010
     12 noon - 1 pm EST or 17:00 - 18:00 (UK); 18:00 - 19:00 (Europe)

     Thursday, May 13, 2010
     9 am - 10 am EST or 14:00 - 15:00 (UK); 15:00 - 16:00 (Europe)

Primary cell culture, over time, has become a major tool in academic research and the biopharmaceutical industry. Obtaining viable culture from a tissue sample and maintaining it for experimental, diagnostic or therapeutic purposes is very challenging. Based on laboratory protocols and several years of practical experience with primary cell culture, this webinar will review methods for initiating primary cell culture and their advantages and limitations. In addition, the information will provide a foundation for individual researchers to experiment, explore, and establish protocols for their specific needs. Topics included in webinar are:

• Selection techniques for targeting specific cell types
• Suggestions for improving culture results
• Optimizing environmental conditions including medium, specialized surface
  

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Life and Death in Vitro – the Evolution of Techniques for Measuring Cell Growth and Toxicity in Culture

     Presented By Mark Rothenberg, Ph.D.

     Tuesday, June 8, 2010
     12 noon - 1 pm EST or 17:00 - 18:00 (UK); 18:00 - 19:00 (Europe)

     Thursday, June 10, 2010
     9 am - 10 am EST or 14:00 - 15:00 (UK); 15:00 - 16:00 (Europe)

This seminar will discuss the development of in vitro techniques for measuring and estimating cell growth and cytotoxicity including direct cell counting, clonogenic assays and microplate-based assays. Also covered will be some of the problems that are typically encountered when trying to measure cytotoxicity using these techniques:

• Timing experiments
• Defining cytotoxicity
• What is the dose?
• Interpreting results
  

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"We are not getting information like this from anywhere. The seminar was amazing, very useful to my work. Thanks for organizing these sessions."

"Great tips! I'll pass along information I learned here to my colleagues to let them know how we should conduct cell culture properly. Many thanks indeed again."

"I use your seminars as training for new employees and estimate they save my company more than $24,000 a year in training costs."

"I have been doing cell culture for 20+ years, and found the presentation informative and thought provoking. One can never learn enough about cell culture as it is an evolving art/science."

Founded in 1925, ATCC is the world's leading Biological Resource Center. Its mission is to acquire, authenticate, preserve, produce, develop and share biological materials for the advancement of scientific knowledge. ATCC offers over 3,600 cell lines from 80 species as well as fully tested media, sera and tissue culture reagents for growing them. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement, validation and application of scientific knowledge.